save “C:Documents and SettingsUserDesktopOutput” “C:Documents and SettingsUserDesktopPanel_1.1”Īrguments: for this test, I just wanted my outputs to be saved to this folder called output “C:Program FilesFlowJo vX10.8.0FlowJo_Vx10.exe” Here is an example: I created a template file and specified and output folder after the template is ran. The arguments below can be used just like the Mac. Now you can specify where your template is located, where your data is located, and what other arguments you would like to include in the command. “C:Program FilesFlowJo Vx10.8.0FlowJo_V10.exe” (quotes are very important in the cmd line for XP) Should look like this when finished: using the tab key filled in most of this for me. Then Flo and hit the tab key till you get to the exe file inside the FlowJo program file. Then Flo and hit the tab key to you get the FlowJo program you are using. So to locate XP’s program files and FJ, type in Command LineĬ:Pro and hit the tab key till Program Files is automatically entered. It’s best to use the tab command to find the right file structure with this OS system. At root folder level and the name of the. You will want to open your Programs Folder and make note of the exact FlowJo name. How you invoke FlowJo depends on the operating system. See below for an example.Ĭommand line FlowJo is invoked by referencing an installed version of FlowJo. Note: You must use quotes to reference any file directories that have spaces in them. If it is a workspace, it is stored in the same folder as the template.
– Imports the specified folder of external CLR files. Output files may be redirected to a specific folder. – Performs automated exporting of FCS, CSV, or CLR files. – Performs automated batching of layouts and tables. – Loads the data into the workspace, assigning samples to the appropriate groups, performing compensation, gating, and statistic calculations as needed. If it is an ACS file, the most recent embedded workspace is used. FlowJo is currently developed by the Ashland, Oregon-based FlowJo LLC, a subsidiary of Becton Dickinson.– Opens the specified template (WSPT), workspace (WSP), or ACS file.
Flowjo linux windows#
In 2002, Tree Star released a Windows version. calcium flux analysis, proliferation analysis, quantification, cluster identification and backgating displayįlowJo became a commercial product in 1996. Thus, you can apply a gate to a sample, copy it to the group, and that gate will be automatically placed on all samples in the group.įlowJo provides tools for the creation of histogram and other plot overlays, cell cycle analysis. When an operation on a group is initiated, FlowJo can perform the same operation on every sample belonging to that group. Within a workspace, samples can be grouped or sorted by various attributes such as the panel of antibodies with which they are stained, tissue type, or patient from whom they came. FlowJo's ability to automate repetitive operations facilitates the production of statistics tables and graphical reports when the experiment involves many samples, parameters and/or operations. Viewing an entire experiment in a Workspace permits organizing and managing complex cytometry experiments and produces detailed graphical reports. In FlowJo, samples are organized in a "Workspace" window, which presents a hierarchical view of all the samples and their analyses (gates and statistics). FlowJo will import and analyze cytometry data regardless of which flow cytometer is used to collect the data. Files produced by modern flow cytometers are written in the Flow Cytometry Standard format with an.
Flowjo linux software#
JSTOR ( October 2009) ( Learn how and when to remove this template message)įlowJo is a software package for analyzing flow cytometry data.Please improve this article by adding secondary or tertiary sources. This article relies excessively on references to primary sources.
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